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We employ a sample of 135 873 RR Lyrae stars (RRLs) with precise photometric-metallicity and distance estimates from the newly calibrated P–ϕ31–R21–[Fe/H] and Gaia G band P–R21–[Fe/H] absolute magnitude–metallicity relations of Li et al., combined with available proper motions from Gaia EDR3, and 6955 systemic radial velocities from Gaia DR3 and other sources, in order to explore the chemistry and kinematics of the halo of the Milky Way (MW). This sample is ideally suited for characterization of the inner- and outer-halo populations of the stellar halo, free from the bias associated with spectroscopically selected probes, and for estimation of their relative contributions as a function of Galactocentric distance. The results of a Gaussian mixture model analysis of these contributions are broadly consistent with other observational studies of the halo, and with expectations from recent MW simulation studies. We apply the hdbscan clustering method to the specific energies and cylindrical actions (E, Jr, Jϕ, Jz), identifying 97 dynamically tagged groups (DTGs) of RRLs, and explore their associations with recognized substructures of the MW. The precise photometric-distance determinations (relative distance errors on the order of 5 per cent or better), and the resulting high-quality determination of dynamical parameters, yield highly statistically significant (low) dispersions of [Fe/H] for the stellar members of the DTGs compared to random draws from the full sample, indicating that they share common star-formation and chemical histories, influenced by their birth environments.

We use 3653 (2661 RRab, 992 RRc) RR Lyrae stars (RRLs) with 7D (3D position, 3D velocity, and metallicity) information selected from Sloan Digital Sky Survey, Large Sky Area Multi-Object Fiber Spectroscopic Telescope, and Gaia EDR3, and divide the sample into two Oosterhoff groups (Oo I and Oo II) according to their amplitude–period behaviour in the Bailey diagram. We present a comparative study of these two groups based on chemistry, kinematics, and dynamics. We find that Oo I RRLs are relatively more metal-rich, with predominately radially dominated orbits and large eccentricities, while Oo II RRLs are relatively more metal-poor, and have mildly radially dominated orbits. The Oosterhoff dichotomy of the Milky Way’s halo is more apparent for the inner-halo region than for the outer-halo region. Additionally, we also search for this phenomenon in the haloes of the two largest satellite galaxies, the Large and Small Magellanic clouds, and compare over different bins in metallicity. We find that the Oosterhoff dichotomy is not immutable, and varies based on position in the Galaxy and from galaxy to galaxy. We conclude that the Oosterhoff dichotomy is the result of a combination of stellar and galactic evolution, and that it is much more complex than the dichotomy originally identified in Galactic globular clusters.

Human gastric pathogenHelicobacter pylori(H. pylori) is the primary risk factor for gastric cancer and is one of the most prevalent carcinogenic infectious agents. Vacuolating cytotoxin A (VacA) is a key virulence factor secreted byH. pyloriand induces multiple cellular responses. Although structural and functional studies of VacA have been extensively performed, the high-resolution structure of a full-length VacA protomer and the molecular basis of its oligomerization are still unknown. Here, we use cryoelectron microscopy to resolve 10 structures of VacA assemblies, including monolayer (hexamer and heptamer) and bilayer (dodecamer, tridecamer, and tetradecamer) oligomers. The models of the 88-kDa full-length VacA protomer derived from the near-atomic resolution maps are highly conserved among different oligomers and show a continuous right-handed β-helix made up of two domains with extensive domain–domain interactions. The specific interactions between adjacent protomers in the same layer stabilizing the oligomers are well resolved. For double-layer oligomers, we found short- and/or long-range hydrophobic interactions between protomers across the two layers. Our structures and other previous observations lead to a mechanistic model wherein VacA hexamer would correspond to the prepore-forming state, and the N-terminal region of VacA responsible for the membrane insertion would undergo a large conformational change to bring the hydrophobic transmembrane region to the center of the oligomer for the membrane channel formation.